Interstitial albumin concentration measured during growth of perivascular cuffs in liquid-filled rabbit lung

The growth rate and albumin concentration of interstitial fluid cuffs were measured in isolated rabbit lungs inflated with albumin solution (3 g/dl) to constant airway (Paw) and vascular pressures for up to 10 h. Cuff size was measured from images of frozen lung sections, and cuff albumin concentration (Cc) was measured from the fluorescence of Evans blue labeled albumin that entered the cuffs from the alveolar space. At 5-cmH2O Paw, cuff size peaked at 1 h and then decreased by 75% in 2 h. The decreased cuff size was consistent with an osmotic absorption into the albumin solution that filled the vascular and alveolar spaces. At 15-cmH2O Paw, cuff size peaked at 0.25 h and then remained constant. Cc rose continuously at both pressures, but was greater at the higher pressure. The increasing Cc with a constant cuff size was modeled as diffusion through epithelial pores. Initial Cc-to-airway albumin concentration ratio was 0.1 at 5-cmH2O Paw and increased to 0.3 at 15 cmH2O, a behavior that indicated an increased permeability with lung inflation. Estimated epithelial reflection coefficient was 0.9 and 0.7, and equivalent epithelial pore radii were 4.5 and 6.1 nm at 5- and 15-cmH2O Paw, respectively. The initial cuff growth occurred against an albumin colloid osmotic pressure gradient because a high interstitial resistance reduced the overall epithelial-interstitial reflection coefficient to the low value of the interstitium.