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Advances in capillary electrophoretically mediated microanalysis
Sigrid Van Dyck1, Eliangiringa Kaale, Sona Nováková
1Laboratorium voor Farmaceutische Chemie en Analyse van Geneesmiddelen, Katholieke Universiteit Leuven, Leuven, Belgium.
Electrophoresis
|December 9, 2003
Summary
This review covers advancements in capillary electrophoresis for studying enzyme reactions and derivatization. It details electrophoretically mediated microanalysis (EMMA) techniques and their applications since 1996.
Area of Science:
- Analytical Chemistry
- Biochemistry
Background:
- Capillary electrophoresis (CE) offers powerful separation capabilities.
- In-line analysis of biochemical processes and sample preparation is increasingly important.
Purpose of the Study:
- To review recent developments in CE for in-line enzyme reactions and derivatization.
- To provide an overview of electrophoretically mediated microanalysis (EMMA) and its applications.
Main Methods:
- Literature review of CE applications in enzyme reactions and derivatization since 1996.
- Classification of EMMA modes (continuous and transient) for enzyme assays.
- Categorization of in-line derivatization protocols based on functional groups.
Main Results:
- EMMA has evolved significantly for studying enzyme kinetics and mechanisms.
- Various enzymes have been successfully analyzed using EMMA-based assays.
- Effective in-line derivatization strategies have been developed for diverse functional groups.
Conclusions:
- CE, particularly EMMA, is a versatile tool for real-time biochemical analysis.
- The reviewed techniques enable efficient and sensitive study of enzyme reactions and sample derivatization.