Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Apoptosis in zebrafish development.

Michiaki Yamashita1

  • 1National Research Institute of Fisheries Science, 2-12-4 Fukuura, Yokohama 236-8648, Japan. mic@affrc.go.jp

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
|December 10, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Determination of Selenoneine in Seafood and Seafood-Derived Products.

Journal of AOAC International·2025
Same author

Selenoneine scavenges hydroxyl radicals by self-oxidation in hydrogen peroxide and by forming seleninic acid, which is reduced back to selenoneine.

Metallomics : integrated biometal science·2025
Same author

Validation of a New Liquid Asymmetric-Electrode Plasma Optical Emission Spectroscopy (LAEP-OES) Method for Measurement of Total Mercury in Tuna.

Journal of AOAC International·2024
Same author

Selenoneine Is Methylated in the Bodies of Mice and then Excreted in Urine as Se-Methylselenoneine.

Biological trace element research·2023
Same author

Special Issue-JSMB Marine Biotechnology 2022 Conference.

Marine biotechnology (New York, N.Y.)·2023
Same author

Distribution and Effects of Selenoneine by Ingestion of Extract from Mackerel Processing Residue in Mice.

Marine biotechnology (New York, N.Y.)·2023

Apoptosis, or programmed cell death, plays a key role in fish development and disease. Zebrafish serve as a valuable model for studying apoptosis in vivo, aiding research into its functions and regulation.

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Cell Biology

Background:

  • Apoptosis (programmed cell death) is crucial for vertebrate development and pathogenesis.
  • Understanding apoptosis in fish development is essential for biological and medical research.
  • Key genes regulating apoptosis, such as caspases, have been identified in various fish species.

Purpose of the Study:

  • To review the prominent features of apoptosis during fish development.
  • To investigate the in vivo functions of apoptosis in development, morphogenesis, and sex differentiation.
  • To explore the relationship between caspase activity and developmental processes.

Main Methods:

  • Cloning of caspases and apoptosis-regulating genes from zebrafish (Danio rerio) and other fish.

Related Experiment Videos

  • Generation of transgenic zebrafish overexpressing procaspase-3 to study caspase-development links.
  • Monitoring stress-induced apoptosis in zebrafish embryos using whole mount TUNEL staining and caspase assays.

    • Main Results:

    • Zebrafish (Danio rerio) possess conserved apoptosis-regulating genes, including caspases.
    • Transgenic zebrafish models allow for the in vivo investigation of procaspase-3 function during development.
    • Established methods enable the detection and analysis of apoptosis in zebrafish embryos.

    Conclusions:

    • Zebrafish are a powerful experimental model for studying the in vivo mechanisms of apoptosis.
    • Apoptosis plays significant roles in fish development, morphogenesis, and sex differentiation.
    • Further research in zebrafish can elucidate the cause-and-effect relationships between caspases and developmental processes.