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Updated: Aug 16, 2026
Analytical Techniques for Assaying Nitric Oxide Bioactivity
Published on: June 18, 2012
Determination of trace concentrations of dissolved nitric oxide in a biological buffer
E A G Demoncheaux1, P J Foster, C D Borland
1Division of Clinical Sciences (South), School of Medicine and Biomedical Sciences, Floor F, University of Sheffield, Beech Hill Road, Sheffield, UK S10 2RX. e.demoncheaux@sheffield.ac.uk
Abstract:
A new real-time method for measuring a trace concentration of nitric oxide (NO) in a complex matrix routinely used in pharmacological studies of its bioactivity is described. NO was quantified as a gas by chemiluminescence after extraction from a continuous liquid sample flow with a limit of detection of 0.042 nmol dm(-3) at a signal to noise ratio of 3. Theories to calculate the concentration of NO in the liquid sample flow from a direct measurement of NO in the extraction carrier gas are presented. The efficiency of extraction is determined by a stopflow experiment. An example is presented of the measurement of the steady-state concentrations of NO in Krebs-bicarbonate buffer at pH 7.4 and 37 degrees C when its liquid surface is sequentially exposed to gases containing various concentrations of NO in O2 plus CO2.
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