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Correlation between p53 mutations and HPV in bilharzial bladder cancer
Hussein M Khaled1, Abeer A Bahnassi, Abdel-Rahman N Zekri
1Medical Oncology Department, Cairo University, Cairo, Egypt.
Urologic Oncology
|December 13, 2003
Summary
p53 gene mutations and human papillomavirus (HPV) were investigated in Bilharzial bladder cancer (BBC). Both factors may independently contribute to BBC development, with distinct mutational patterns compared to Western bladder cancers.
Area of Science:
- Oncology
- Virology
- Genetics
Background:
- Alterations in the p53 tumor suppressor gene are common in human cancers, including bladder cancer.
- Human papillomavirus (HPV) infection has been linked to various urological malignancies.
Purpose of the Study:
- To investigate p53 gene status and HPV frequency in Bilharzial bladder cancer (BBC) in Egypt.
- To correlate these findings with clinicopathological features.
Main Methods:
- Screening of p53 gene mutations (exons 4-10) using SSCP and sequencing.
- Detection of p53 protein overexpression via immunohistochemistry (IHC).
- Identification and typing of HPV-DNA using PCR.
Main Results:
- p53 mutations were found in 33.3% and overexpression in 35.6% of BBC cases.
- HPV-DNA was detected in 48.97% of cases, predominantly HPV type 16.
- Significant correlations were observed between p53 mutation and pathological stage, and p53 overexpression and tumor grade.
Conclusions:
- The mutational spectrum in BBC differs from Western bladder cancers.
- HPV may play an etiological role in BBC.
- Both HPV infection and p53 gene abnormalities may contribute independently to Bilharzial bladder carcinogenesis.