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Methods for Facilitating Microbial Growth on Pulp Mill Waste Streams and Characterization of the Biodegradation Potential of Cultured Microbes
Published on: December 12, 2013
Preliminary studies on bacterial decolorization of H-acid based azo dye-reactive black 5
Sagarika Mohanty1, Nageswara Rao Neti, Santosh Narain Kaul
1National Environmental Engineering Research Institute, Nehru Marg, Nagpur--440020, India.
Abstract:
A co-culture acclimatized to H-acid was used to degrade Reactive Black 5 (RB 5), a bis azo dye having central H-acid function. The effect of substrate concentration, pH and medium composition on the decolorization has been investigated. Decolorization was found independent of pH. Luria-Bertani broth favored decolorization over Yeast Extract; however further decolorization experiments have been conducted using Yeast Extract. The Michaelis-Menten Kinetic model is found to describe the dependence of specific decolorization rate on the RB 5 dye concentration.
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