Related Experiment Video
Updated: Aug 29, 2026
High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
Published on: January 21, 2015
Characterization of human cervical precancerous tissue through the fourier transform infrared microscopy with mapping
Jui-I Chang1, Yaw-Bin Huang, Pao-Chu Wu
1Department of Obstetrics and Gynecology, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung, Taiwan, Republic of China.
Objective:
The aim of this study was to evaluate directly the utility of Fourier transform infrared microscopy (FTIR-MC) in detecting cervical precancer by comparison with the pathological examination of biopsy tissue.
Methods:
Cervical biopsy specimens were obtained from the outpatient department at Kaohsiung Municipal Ta-Tung Hospital. Freshly isolated tissue was washed in isotonic saline and then frozen for use. Two successive slices were cut with a microtome. One unstained slice was used for reflectance FTIR-MC analysis; the other slice was stained with hematoxylin and eosin and used for position reference by microscopic mapping.
Results:
The spectral region from 950 to 1500 cm(-1) is the most critical region in the IR spectrum of the tissue. There were two special peaks that had growth and decline in the IR spectra. The one at 1150 cm(-1) disappeared and the other at 1240 cm(-1) broadened when the tissue had pathological changes. From the results, we found that the ratio of the areas of the two regions between 1130 to 1180 cm(-1) and 1180 to 1260 cm(-1) was an exceptionally useful factor in discriminating precancerous tissues from normal tissues.
Conclusion:
We carried out microscopic mapping of the tissues and demonstrated that the color map reflects pathological changes in the cervical tissues.
