Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ultracompact autocorrelator for multiphoton microscopy.

F Quercioli1, A Ghirelli, B Tiribilli

  • 1Istituto Nazionale di Ottica Applicata, Biophotonics Laboratory, Largo Enrico Fermi 6, 50125 Firenze, Italy. quercioli@inoa.it

Microscopy Research and Technique
|December 17, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

MRI approach to the patient with suspected dementia: artificial intelligence techniques and semi-quantitative rating scales compared.

Frontiers in radiology·2026
Same author

Contact-free characterization of nuclear mechanics using correlative Brillouin-Raman Micro-Spectroscopy in living cells.

Acta biomaterialia·2025
Same author

Robustness analysis of surface-guided DIBH left breast radiotherapy: personalized dosimetric effect of real intrafractional motion within the beam gating thresholds.

Strahlentherapie und Onkologie : Organ der Deutschen Rontgengesellschaft ... [et al]·2023
Same author

Surface-guided DIBH radiotherapy for left breast cancer: impact of different thresholds on intrafractional motion monitoring and DIBH stability.

Strahlentherapie und Onkologie : Organ der Deutschen Rontgengesellschaft ... [et al]·2022
Same author

Acoustofluidic interferometric device for rapid single-cell physical phenotyping.

European biophysics journal : EBJ·2022
Same author

A versatile and compact surface plasmon resonance spectrometer based on single board computer.

The Review of scientific instruments·2020
Same journal

Deep Learning Based Framework for Detection and Classification of Leukemia Using Microscopic Images.

Microscopy research and technique·2026
Same journal

Externally Controlled In Situ SEM: Multi-Rate Scanning With Signal Regulation and Spatiotemporal Fusion.

Microscopy research and technique·2026
Same journal

In Situ TEM Observation of Phase Transformation Nucleation at the Near-Surface of Synthetic Aragonite.

Microscopy research and technique·2026
Same journal

Morpho-Anatomical and HPTLC Investigations of Lysimachia nummularia L. (Primulaceae) Grown in Switzerland.

Microscopy research and technique·2026
Same journal

Macroscopic, Histological and Ultrastructural Features of the Tongue of the Anatolian Wild Boar (Sus scrofa libycus).

Microscopy research and technique·2026
Same journal

Ultrastructural Insights Into the Reproductive Anatomy and Eggs of Cotton Pink Bollworm, Pectinophora gossypiella Saunders (Lepidoptera: Gelechiidae).

Microscopy research and technique·2026
See all related articles

Characterizing laser pulses is crucial for multiphoton microscopy. This study presents a simple, ultracompact autocorrelator for measuring pulse width directly within the microscope setup.

Area of Science:

  • Optics and Photonics
  • Biomedical Imaging
  • Laser Physics

Background:

  • Accurate pulse temporal characterization is essential for optimizing ultrafast laser systems, particularly in multiphoton microscopy.
  • Ti:Sapphire lasers are widely used for their ultrashort pulse generation capabilities in demanding imaging applications.

Purpose of the Study:

  • To develop and demonstrate an ultracompact autocorrelator for in-situ pulse width measurements.
  • To simplify the process of pulse temporal characterization at the focal plane of a microscope objective.

Main Methods:

  • An ultracompact autocorrelator setup was designed and integrated.
  • A simple measurement procedure was established utilizing the existing confocal microscope's detection and data processing capabilities.
  • No additional specialized instrumentation beyond basic optical elements was required.

Related Experiment Videos

Main Results:

  • The autocorrelator successfully performed pulse width measurements directly at the focal plane.
  • The procedure eliminated the need for external pulse characterization equipment.
  • The integrated system leveraged the microscope's inherent functionalities for data acquisition and analysis.

Conclusions:

  • This method offers a streamlined and efficient approach for pulse temporal characterization in multiphoton microscopy.
  • The reported technique simplifies experimental setups and reduces operational complexity for ultrafast laser microscopy.
  • The findings contribute to improved usability and performance of Ti:Sapphire laser systems in biological imaging.