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Updated: Aug 29, 2026

Fluorescent Silver Staining of Proteins in Polyacrylamide Gels
Published on: April 21, 2019
Chromium (III) enhanced diamine silver staining of proteins and DNA in gels
Zhi-dong Zhou1, Wang-yi Liu, Min-qian Li
1State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031, PR China.
Abstract:
Chromium (III) enhanced the sensitivities of diamine silver staining of four proteins between 6- and 50-fold over that of the Coomassie Brilliant Blue (CBB)-chromium modified thiosulfate-silver staining method (Zhou et al. Biotechnology Letters, 2002, 24: 1561-1567). Using six dsDNA fragments, the detection limits of this new method was 10 to 30 pg per band, being 10- to 25-fold more sensitive than previous methods.
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