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[Cytoxic T lymphocyte responses to hepatitis B virus small surface antigen in BALB/c mice]
Li Shi1, Shu-ren Liu, Xue-rui Yi
1Center for Hepatic Diseases of PLA, 458 Hospital of PLA, Guangzhou 510602, China.
Objective:
To observe cytotoxic T lymphocytes (CTL) responses induced by immunization of mice with hepatis B small surface antigen (S-HBsAg).
Methods:
BALB/c(H-2d) mice were injected intraperitoneally with S-HBsAg at the doses ranging from 0-5 microg with a booster injection 2 weeks later. Four weeks after the booster immunization, the specific cytotoxic reactivity of the spleen lymphocytes isolated from the immunized mice, following stimulation by S-HBsAg specific CTL epitope peptide in vitro, with 51Cr-labeled P815 cells treated with the peptide was observed.
Results:
The percentage of specific release of 51Cr in mice immunized with S-HBsAg at the doses of 0, 0.65, 1.25, 2.5, and 5 microg was 31.21%+/-9.23%, 42.36%+/-19.32%, 91.21%+/-22.97%, 69.25%+/-24.13% and 51.49%+/-21.661% respectively. In mice receiving the only primary vaccination, the corresponding percentage was 27.34%+/-14.25%, 32.27%+/-15.35%, 56.28%+/-24.35%, 44.34%+/-18.65% and 40.76+/-56% respectively.
Conclusion:
Immunization with S-HBsAg efficiently elicits CTL response in BALB/c mice in a dose-dependent manner and enhanced by booster immunization.
