Related Experiment Video
Updated: Aug 29, 2026
Transduction-Transplantation Mouse Model of Myeloproliferative Neoplasm
Published on: December 22, 2016
[The formation of transgenic mice with hemangiomas]
Qing Xu1, Zhi-yuan Zhang, Wan-tao Chen
1Department of Oral and Maxillofacial Surgery, School of Stomatology, Shanghai Second Medical University, Shanghai 200011, China.
Objective:
To set up the animal model of hemangioma by microinjecting the PyMT transgenic DNA.
Methods:
Constructing the transgenic PyMT gene, and microinjecting it into fertilized embryos which were transferred to pseudopregnant recipients then. Observing the phenotype of the newborn-mice, detecting the integration of transgenic DNA by PCR, and analyzing the histological morphon of the neoplasm of the mice.
Results:
The transgenic DNA was proved to be right and has been microinjected into 579 fertilized embryos, 62 mice were born. Within the 62 mice, one mouse was found being the phenotype of hemangioma. PyMT gene was expressed in the total DNA of the transgenic mouse by PCR.
Conclusion:
It could be a good way to build animal model of hemangioma with transgenic PyMT DNA.
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