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The Centre for Modeling Human Disease Gene Trap resource.

Christine To1, Trevor Epp, Tammy Reid

  • 1Centre for Modeling Human Disease, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada.

Nucleic Acids Research
|December 19, 2003
PubMed
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Gene trap mutagenesis creates random mutations in mouse cells, identified by sequence tags. This resource aids researchers in understanding gene function and annotating the mammalian genome.

Area of Science:

  • Genetics
  • Genomics
  • Developmental Biology

Background:

  • Gene trap mutagenesis is a method to generate random loss-of-function mutations in mouse embryonic stem cells.
  • These mutations can be identified by sequence tags and often report endogenous gene expression patterns.

Purpose of the Study:

  • To create a valuable resource of new mouse mutations for the scientific community.
  • To functionally annotate the mammalian genome and aid in identifying candidate genes from phenotype-driven screens.

Main Methods:

  • Performing expression- and sequence-based screens of gene trap insertions.
  • Utilizing multiplexed in vitro differentiation and induction assays.
  • Generating sequence tags to complement gene expression profiles.

Related Experiment Videos

Main Results:

  • A searchable online database of gene trap insertions is available (http://www.cmhd.ca/sub/genetrap.asp).
  • Researchers can search for insertions based on gene expression in specific cell lineages, in vitro conditions, or sequence identity.

Conclusions:

  • The generated gene trap insertion clones serve as a valuable resource for researchers worldwide.
  • This resource facilitates the functional annotation of the mammalian genome and the identification of genes associated with specific phenotypes.