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[Hypodense eosinophils in blood of pediatric patients with asthma]
1Médico Pneumopediatra do Hospital de Clínicas, PAG UFPR, Curitiba.
Objective:
Determine the number of circulating hypodense eosinophils in the blood of atopic asthmatics.
Methods:
The hypothesis was that hypodense eosinophils, the activated cells, were higher in asthmatics than in non-atopic children. Granulocytes were separated from mononuclear cells by Ficoll-hypaque. Gradients of density were generated through dilutions of Percoll for fractionations of cells.
Results:
Asthmatics had blood eosinophilia (768 +/- 75/mcl) when compared to normal control (200 +/- 28/mcl), and higher mean hypodense eosinophils (39%) as compared to normal children (11.7%).
Conclusion:
Eosinophils accumulate in asthmatic, especially the low density activated variant, suggesting its active role in the allergic inflammatory process.
