Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Phage integrases: biology and applications.

Amy C Groth1, Michele P Calos

  • 1Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305-5120, USA.

Journal of Molecular Biology
|December 23, 2003
PubMed
Summary

Phage integrases, enzymes for DNA recombination, are crucial tools for genetic engineering in eukaryotic cells. Serine integrases show particular promise for applications in gene therapy and creating transgenic organisms.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Expression of the <i>odd-2</i> Gene in <i>C. elegans</i>.

microPublication biology·2023
Same author

The <i>odd-1(tm848)</i> mutation has no significant effect on brood size in <i>Caenorhabditis elegans</i>.

microPublication biology·2021
Same author

Knock-in Blunt Ligation Utilizing CRISPR/Cas9.

Bio-protocol·2021
Same author

Nucleofection of phiC31 Integrase Protein Mediates Sequence-Specific Genomic Integration in Human Cells.

Journal of molecular biology·2020
Same author

Plasmid-Mediated Gene Therapy in Mouse Models of Limb Girdle Muscular Dystrophy.

Molecular therapy. Methods & clinical development·2020
Same author

Lack of RAC.

Molecular therapy : the journal of the American Society of Gene Therapy·2018

Area of Science:

  • Molecular Biology
  • Enzymology
  • Genetics

Background:

  • Phage integrases are enzymes catalyzing site-specific DNA recombination.
  • They are classified into tyrosine and serine families based on catalytic mechanisms.
  • Serine integrases are efficient and do not require host cofactors, unlike tyrosine integrases.

Purpose of the Study:

  • To highlight the significance of phage integrases in genetic manipulation of eukaryotic cells.
  • To discuss the advantages of serine integrases for genomic applications.
  • To explore the potential of directed evolution for enhancing integrase function.

Main Methods:

  • Review of existing literature on phage integrase mechanisms and applications.
  • Comparison of tyrosine and serine integrase families.
  • Discussion of experimental findings on serine integrase activity in mammalian cells.

Main Results:

  • Phage integrases enable precise recombination between attP and attB sites.
  • Serine integrases are effective in eukaryotic cells, including mammalian systems.
  • Partial sequence identity allows for integration into native genomic sites.

Conclusions:

  • Phage integrases, particularly serine recombinases, offer powerful tools for precise genome engineering in eukaryotes.
  • Applications include gene therapy, transgenic organism creation, and cell line engineering.
  • Directed evolution can further expand their utility for genomic modification.

Related Experiment Videos