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Updated: Aug 29, 2026

Visualization of Bacterial Toxin Induced Responses Using Live Cell Fluorescence Microscopy
Published on: October 1, 2012
Activation of Serratia marcescens hemolysin through a conformational change
Georg Walker1, Ralf Hertle, Volkmar Braun
1Mikrobiologie/Membranphysiologie, Universität Tübingen, Tübingen, Germany.
Abstract:
For Serratia marcescens, secreted hemolysin/cytotoxin is not only secreted but also activated by an outer membrane protein. Excluding posttranslational processing by mass spectrometry, the conformation of active and inactive ShlA derivatives strongly differed in electrophoretic mobilities, gel permeation chromatography, sensitivity to trypsin, circular dichroism, and intrinsic fluorescence. We concluded that ShlB interacts with ShlA during secretion and imposes a conformational change in ShlA to form the active hemolysin.
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