Related Experiment Video

Updated: Aug 29, 2026

Homemade Site Directed Mutagenesis of Whole Plasmids
07:11

Homemade Site Directed Mutagenesis of Whole Plasmids

Published on: May 11, 2009

A one-tube, two-step polymerase chain reaction-based site-directed mutagenesis method with simple identification of

Zsolt B Nagy1, Ferenc Felföldi, László Tamás

  • 1Laboratory of Functional Genomics, Biological Research Centre, Hungarian Academy of Sciences, P.O. Box 521, Szeged H-6701, Hungary.

Analytical Biochemistry
|December 24, 2003
PubMed
Abstract

No abstract available in PubMed .

More Related Videos

Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli
07:04

Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli

Published on: February 5, 2019

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
08:23

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

Related Experiment Videos

Last Updated: Aug 29, 2026

Homemade Site Directed Mutagenesis of Whole Plasmids
07:11

Homemade Site Directed Mutagenesis of Whole Plasmids

Published on: May 11, 2009

Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli
07:04

Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli

Published on: February 5, 2019

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
08:23

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

Related Concept Videos

PCR02:07

PCR

The polymerase chain reaction, or PCR, is a widely used technique for copying segments of DNA. Due to exponential amplification, PCR can produce millions or billions of DNA copies within just a few hours. In a PCR reaction, a heat-resistant DNA polymerase enzyme amplifies the original DNA through a series of temperature changes inside an automated machine called a thermocycler.PCR is a Versatile Method that Revolutionized Molecular BiologyKary Mullis developed PCR in 1983, for which he was...
PCR - Polymerase Chain Reaction02:07

PCR - Polymerase Chain Reaction

The polymerase chain reaction, or PCR, is a widely used technique for copying segments of DNA. Due to exponential amplification, PCR can produce millions or billions of DNA copies within just a few hours. In a PCR reaction, a heat-resistant DNA polymerase enzyme amplifies the original DNA through a series of temperature changes inside an automated machine called a thermocycler.PCR is a Versatile Method that Revolutionized Molecular BiologyKary Mullis developed PCR in 1983, for which he was...

Articles linked to this work by shared authors, journal, and citation graph.

SPOCK1 overexpression suggests poor prognosis in head and neck squamous cell carcinomas.

Pathology oncology research : POR·2026

[Vestibulocochlear infarct: a challenging diagnosis].

Orvosi hetilap·2026

Our experience with super-thin anterolateral thigh flap for intraoral reconstruction.

Oral and maxillofacial surgery·2026

Amyloid-associated cognitive trajectories in aging dogs mirror early changes in humans.

Neurobiology of aging·2026

Tilorone suppresses TGF-β-driven fibroblast activation and restores BMP-Smad1/5/8 signaling: implications for laryngotracheal fibrosis.

Journal of translational medicine·2026

Post-Radiation PET Shows Higher Diagnostic Accuracy in HPV-Negative Head and Neck Cancers.

Cancers·2026

Fe-Co bimetallic nanozyme-amplified lateral-flow immunoassay for the sensitive detection of porcine rotavirus.

Analytical biochemistry·2026

Integrated sustainability evaluation of electrochemical quantification of flibanserin and caffeine in various matrices.

Analytical biochemistry·2026

A NanoBiT-based high-throughput assay system for monitoring osteoclast fusion.

Analytical biochemistry·2026

Integrating artificial intelligence with spectrophotometry: A novel python framework for automated green assessment and pharmaceutical analysis.

Analytical biochemistry·2026

HF-MMLLE using natural deep eutectic solvents for determination of potential lung cancer biomarkers in urine.

Analytical biochemistry·2026

Best practices for isothermal titration calorimetry of protein-DNA interactions.

Analytical biochemistry·2026

VMP1 restricts STING trafficking and activation through controlling CDIPT recruitment to ER membrane curvature.

Cell death and differentiation·2026

Disruption of Chlamydia trachomatis Essential Genes Using DOPE (Dependence on Plasmid-Mediated Expression).

Methods in molecular biology (Clifton, N.J.)·2026

In Silico Design of Amino Acid-Functionalized Materials for the Capture of Neonicotinoids.

Journal of computational chemistry·2026

DELLA proteins enhance cadmium tolerance by coordinating redox protection and cadmium partitioning in Arabidopsis.

Planta·2026

ACE2 dysregulation and lipid metabolism: Mechanistic interplay between COVID-19 and neurodegeneration.

Molecular aspects of medicine·2026

Covalent allosteric inhibition of AARS1 lactyltransferase.

Nature communications·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies
Jove
Visualize
Contact Us