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Related Experiment Videos

Well diffusion for antifungal susceptibility testing.

S Magaldi1, S Mata-Essayag, C Hartung de Capriles

  • 1Instituto de Medicina Tropical, Universidad Central de Venezuela, Caracas, Venezuela.

International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases
|December 24, 2003
PubMed
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The well diffusion test offers a reliable and cost-effective alternative for antifungal susceptibility testing of Candida species. This method shows good correlation with the NCCLS standard, aiding in treatment decisions for resistant infections.

Area of Science:

  • Medical Mycology
  • Antimicrobial Resistance
  • Clinical Microbiology

Background:

  • Rising resistance of Candida species to antifungal drugs poses a significant clinical challenge.
  • Antifungal susceptibility testing is crucial for patients with treatment failures before empirical therapy selection.

Purpose of the Study:

  • To evaluate the well diffusion (WD) test as a potential alternative for antifungal susceptibility testing of Candida species.
  • To compare the efficacy of the WD test against the NCCLS standard broth microdilution method.

Main Methods:

  • One hundred fifty-eight Candida isolates were tested using both broth microdilution (NCCLS standard) and well diffusion testing.
  • Antifungal agents included fluconazole, itraconazole, posaconazole, caspofungin, and amphotericin B.

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Main Results:

  • No significant difference (p>0.05) was found between WD and NCCLS methods for most Candida species and antifungal agents.
  • Significant differences were observed for fluconazole in Candida glabrata and Candida albicans, indicating potential variations in specific scenarios.

Conclusions:

  • The well diffusion test is a simple, inexpensive, and reproducible method for antifungal drug susceptibility testing.
  • WD may serve as a viable alternative to the NCCLS method, especially in resource-limited laboratories.