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[Effects of beta-asaron on gene expression in mouse brain]
Yongqi Fang1, Ling Li, Qiduan Wu
1First Affiliated Hospital, Guangzhou University, TCM, Guangzhou 510405.
Objective:
To observe effects of beta-asaron on gene expression in BALB/c mouse brain by gene chip.
Methods:
The mRNA of mouse brain samples of experiment group and control group was markered with cy5, cy3 fluorescence separately, and hybridized with 4000 genes on MGEC-40S gene chip after mix. Fluorescence intensity was scanned with Genepix 400 B scanner and analysed with Genepix Pro3.0 soft.
Results:
Among 4000 genes, there are 15 genes with difference expression (the ratio > 1.9 or < 0.59) in two groups. In beta-asaron group, 4 genes expression raised relating to ion channels, substance transform through membrane, calcium-dependent protein kinase regulation, apoptosis, 11 genes expression declined relating to excitatory neurotransmitter metabolism in the CNS, T lymphocyte tend, genes expression regulation and drug metabolism.
Conclusion:
beta-asaron has effects on multi-target genes in mouse brain.