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Two-dimensional microfabricated sources for nanoelectrospray.
Séverine Le Gac1, Steve Arscott, Cécile Cren-Olivé
1Laboratoire de Chimie Organique et Macromoléculaire, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq, France.
Journal of Mass Spectrometry : JMS
|December 30, 2003
Summary
Researchers developed a novel 2D nanoelectrospray ionization emitter with a nib shape, offering a stable and reliable alternative to traditional needle tips for peptide analysis in mass spectrometry.
Area of Science:
- Analytical Chemistry
- Mass Spectrometry
- Nanotechnology
Background:
- Conventional needle-like nanoelectrospray ionization (NSI) emitters lack reproducibility and robustness.
- NSI emitters are incompatible with high-throughput analysis.
- A planar emitter design offers potential advantages.
Purpose of the Study:
- To explore a novel two-dimensional (2D) nanoelectrospray ionization emitter tip with a nib shape.
- To evaluate the performance of this micro-nib design as an alternative to standard emitter tips.
- To assess its suitability for peptide analysis in mass spectrometry.
Main Methods:
- Fabrication of micro-nib emitter prototypes using microtechnology and SU-8 photoresist.
- Testing of micro-nib sources in mass spectrometry experiments using an ion trap mass spectrometer.
- Analysis of standard peptide samples at concentrations down to 1 micro M.
Main Results:
- Two series of micro-nib sources were fabricated, with the second series featuring an 8 micrometer nib tip width.
- The micro-nib sources demonstrated stable electrospray and reliable peptide analysis.
- Obtained mass spectra showed comparable signal intensity and signal-to-noise ratio to standard emitter tips.
Conclusions:
- The novel 2D micro-nib emitter is a viable and promising alternative to conventional NSI tips.
- This design offers improved robustness and potential for high-throughput analysis.
- The micro-nib emitter provides reliable and sensitive peptide detection in mass spectrometry.