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Updated: Aug 29, 2026

A Modified Surgical Technique for Kidney Transplantation in Mice
Published on: July 22, 2022
The modification of renal transplantation with the usage of own polyethylene receptacle
Artur Pupka1, Paweł Chudoba, Dariusz Patrzałek
1Department of Vascular, General and Transplantological Surgery, Wrocław University of Medicine. apupka@chirn.am.wroc.pl
Abstract:
In this study a method of elimination of the second warm ischemia is shown. The method is based on the application of a specially constructed polyethylene bag, in which the transplanted kidney is placed in the time course from a removal from ice to the reconstruction of vessel flow. The bag is built of polyethylene foil HDPE of low density produced under high pressure. Own construction of the bag (three spaces and polyethylene) enables the storage of a transplanted organ at the stable temperature +4 Celsius degrees. Thanks to the separation of containers for melting ice and for the kidney, possible becomes unrestrained performance of both venous and arterial anastomosis independently of existing operative conditions. Due to the applied method of the elimination of the second warm ischemia with the usage of own construction of polyethylene bag HDPE, one can expect better early renal function after transplantation--decrease in the number of cases and shortening of the time of acute tubular necrosis (ATN--Acute Tubular Necrosis).
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Kidney Transplant I: Introduction
Kidney Transplant II: Surgical Procedure

