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Interaction assay of oligosaccharide with lectins using glycosylasparagine

Mamoru Mizuno1, Midori Noguchi, Mie Imai

  • 1The Noguchi Institute, 1-8-1, Kaga, Itabashi-ku, 173-0003, Tokyo, Japan. mmizuno@noguchi.or.jp

Bioorganic & Medicinal Chemistry Letters
|December 31, 2003
PubMed

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Oligosaccharide Assembly01:24

Oligosaccharide Assembly

Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...