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Fluorescence detection of guanine-adenine transition by a hydrogen bond forming small compound
Keitaro Yoshimoto1, Chun-Yan Xu, Seiichi Nishizawa
1Department of Chemistry, Graduate School of Science, Tohoku University, Aoba-ku, Sendai 980-8578, Japan.
Abstract:
In combination with abasic site-containing oligodeoxynucleotides, 2-amino-4-oxopteridine (pterin) can selectively recognize guanine base over other nucleobases accompanied by fluorescence quenching, which allows clear detection of a guanine-adenine transition with the naked eye.
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