Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Recent developments in the analysis of protein complexes.

Andrzej Dziembowski1, Bertrand Séraphin

  • 1Equipe Labelisée 'La Ligue', Centre de Génétique Moléculaire, CNRS UPR 2167 Associated with University Paris 6, Avenue de la Terrasse, 91198 Cedex, Gif sur Yvette, France.

FEBS Letters
|January 7, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

TENT-5 polyadenylates and regulates male-specific transcripts in Caenorhabditis elegans.

G3 (Bethesda, Md.)·2026
Same author

Degron models: a toolbox for rapid in vivo depletion of essential proteins regulating mRNA metabolism.

Communications biology·2026
Same author

DIS3 mutations enhance AID-driven translocations during B-cell activation, promoting transformation to multiple myeloma.

Nature communications·2026
Same author

PolyA tail segmentation improves the stability of the template DNA and increases the translatability of in vitro transcribed mRNA.

Nucleic acids research·2026
Same author

The CCR4-NOT complex: a multifaceted sensor of molecular signals instructing eukaryotic mRNA translation and stability.

Nucleic acids research·2025
Same author

Polyadenylation landscape of in vivo long-term potentiation in the rat brain.

RNA (New York, N.Y.)·2025

Recent advances in protein purification and mass spectrometry enable high-throughput proteome-wide analysis of protein complexes. Understanding complex function requires further investigation into subunit arrangement and detailed structural analyses.

Area of Science:

  • Biochemistry
  • Proteomics
  • Structural Biology

Background:

  • Protein complexes are crucial for cellular functions.
  • Understanding their composition and structure is key to elucidating biological mechanisms.

Purpose of the Study:

  • To review technical developments enhancing the biochemical characterization of protein complexes.
  • To highlight advancements enabling proteome-wide analyses.
  • To propose future directions for structural and functional studies.

Main Methods:

  • Review of recent technical developments in protein purification (e.g., improved tags, generic strategies).
  • Analysis of advancements in mass spectrometry (sensitivity, automation).
  • Discussion of high-throughput and proteome-wide analysis approaches.

Related Experiment Videos

Main Results:

  • Technical improvements facilitate the purification of diverse protein complexes.
  • Increased mass spectrometry capabilities enable large-scale proteomic studies.
  • Current methods allow for proteome-wide identification of protein complex composition.

Conclusions:

  • High-throughput biochemical characterization of protein complexes is now feasible.
  • Knowledge of composition alone is insufficient for functional understanding.
  • Defining internal subunit arrangement is the next critical step towards detailed structural analysis and functional insights.