Jove
Visualize
Contact Us

Related Experiment Videos

Ordered partitioning reveals extended splice-site consensus information.

Michael Weir1, Michael Rice

  • 1Department of Biology, Wesleyan University, Middletown, Connecticut 06459, USA. mweir@wesleyan.edu

Genome Research
|January 7, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Implementing the Safer Baby Bundle for stillbirth prevention across Queensland maternity services using a modified breakthrough series collaborative.

Implementation science communications·2026
Same author

Artificial Intelligence Analysis of Endoscopy Outperforms Conventional Endoscopic Scoring for Agreement With Symptoms and Quality of Life in a Phase 3 Ulcerative Colitis Trial.

The American journal of gastroenterology·2025
Same author

A novel, standardised approach to balancing effectiveness, efficiency and utility of surveillance AI prediction models for hospitalised patients using sepsis prediction as an exemplar.

Journal of the American Medical Informatics Association : JAMIA·2025
Same author

Artificial Intelligence for Quantifying Endoscopic Mucosal Ulceration in Crohn's Disease.

Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association·2025
Same author

Nipple Areola Complex Reconstruction: An 8-Year Experience With Modified Technique and Systematic Review.

Eplasty·2024
Same author

A History of Channel Coding in Aeronautical Mobile Telemetry and Deep-Space Telemetry.

Entropy (Basel, Switzerland)·2024
Same journal

High-accuracy SNV calling for bacterial isolates using deep learning with AccuSNV.

Genome research·2026
Same journal

Complete sequencing of medaka genomes reveals the architecture of centromeric satellites, giant mobile elements, and sex chromosomes.

Genome research·2026
Same journal

Convergence and conflict among telomere specialized transposons across 60 million years of Drosophilid evolution.

Genome research·2026
Same journal

A unified analysis of cell type- and trajectory-associated pathways in single-cell data using Phoenix.

Genome research·2026
Same journal

Resf1 is required for proper placental development and configuration of trophoblast cell-specific heterochromatin.

Genome research·2026
Same journal

Telomere-driven replicative crisis is driven by large-scale changes in genomic architecture.

Genome research·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Researchers analyzed 10,057 Drosophila splice sites, finding a strong correlation between intron/exon size and sequence conservation. This allowed determination of consensus sequences and revealed long-range effects on spliceosome function.

Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Splice sites are crucial for gene expression, but their sequence characteristics are not fully understood.
  • Large-scale genomic data provides an unprecedented opportunity to study splice site properties.

Purpose of the Study:

  • To analyze a large dataset of Drosophila splice sites to understand sequence conservation and its relationship with intron/exon lengths.
  • To identify consensus sequences at donor and acceptor sites and explore factors influencing spliceosome function.

Main Methods:

  • Computed 10,057 Drosophila splice sites using cDNA and genomic data.
  • Performed information-theoretic analysis of nucleotide sequences surrounding splice sites.
  • Correlated sequence information with intron and exon lengths.

Related Experiment Videos

Main Results:

  • A strong correlation was found between intron/exon sizes and sequence information (conservation).
  • Extensive consensus sequences were determined for longer introns, with refined sequences for shorter introns.
  • Long-range effects influencing spliceosome function across multiple introns were observed.

Conclusions:

  • Splice site sequence conservation is strongly linked to intron and exon lengths.
  • The findings provide insights into spliceosome function and sequence preferences.
  • The analytical approach is valuable for large genomic datasets.