[Immunophenotyping in diagnosis of chronic lymphoproliferative diseases]

With due respect to their many-year experience, the authors focused their attention on the peculiarities of implementation and registration aspects of the immunofluorescence method of immunotyping made in cell suspensions and in tissue imprints; additionally they made a system of a set of monoclonal antibodies (used for the purpose), which enable the differential diagnosis of reactive conditions related with various malignant lymphoproliferative diseases (LPD). It order to specify a nature of lymphoid cells it is suggested to undertake the immunophenotyping withing a gradually expanding set of monoclonal antibodies, which reflects different parameters of lymphoid cells like linear attributes, clonal characteristics, differential-diagnostic markers, functional status and proliferative activity. Typical marker phenotypes of lymphoid cells observed in the main B- and T-cell LPDs are described; a possibility is mentioned that there can be errors in interpreting the phenotyping results while diagnosing an LPD.