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Updated: Aug 29, 2026
Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
Published on: February 1, 2019
In vitro transfection of the human vas deferens using DNA-liposome and DNA-neutral lipid complexes
Pedro Esponda1, Marc Goldstein, Steve S Witkin
1Centro de Investigaciones Biológicas, CSIC, Madrid, Spain. esponda@cib.csic.es
Objective:
To transfect the human vas deferens in vitro.
Design:
Prospective study, description of a procedure.
Setting:
Research center and university hospital.
Patient(S):
Seven fertile men undergoing vasectomies or vasovasostomies.
Intervention(S):
Human vas deferens pieces were transfected in vitro using the p-GeneGrip gene construction, which codifies for the green fluorescent protein (GFP). Lipofectamine or GenePorter were employed as gene vectors.
Main Outcome Measure(S):
After vas deferens epithelium transfection, we described the vas deferens foreign gene expression. Maximum transfection occurred in 14.7% of the vas deferens epithelial cells. After using GenePorter, we observed green fluorescent protein gene expression in 40% of samples, which occupied 9.86% of the epithelial area. After Lipofectamine treatment, transgene expression occurred in 33% of the samples and occupied 9.05% of the epithelial area.
Conclusion(S):
The human vas deferens epithelium has the potential to be modified by gene transfection.
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