Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rapidly developing cocaine-induced peripheral portal liver damage.

S J Charles1, C J Powell

  • 1DH Department of Toxicology, St Bartholomews Hospital Medical College, London, UK.

Toxicology Letters
|December 1, 1992
PubMed
Summary

Cocaine causes rapid liver damage in mice within 2 hours, indicated by cell necrosis and elevated liver enzymes. Prompt medical attention is crucial for suspected cocaine overdose.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Blocking mu-opioid receptors inhibits social bonding in rituals.

Biology letters·2020
Same author

Comparisons of Analytical Approaches for Determining Shell Thicknesses of Core-Shell Nanoparticles by X-ray Photoelectron Spectroscopy.

The journal of physical chemistry. C, Nanomaterials and interfaces·2018
Same author

Effective attenuation lengths for quantitative determination of surface composition by Auger-electron spectroscopy and X-ray photoelectron spectroscopy.

Journal of electron spectroscopy and related phenomena·2017
Same author

Calculations of Electron Inelastic Mean Free Paths. XI. Data for Liquid Water for Energies from 50 eV to 30 keV.

Surface and interface analysis : SIA·2017
Same author

Evaluation of Two Methods for Determining Shell Thicknesses of Core-Shell Nanoparticles by X-ray Photoelectron Spectroscopy.

The journal of physical chemistry. C, Nanomaterials and interfaces·2017
Same author

Evaluating the Internal Structure of Core-Shell Nanoparticles Using X-ray Photoelectron Intensities and Simulated Spectra.

The journal of physical chemistry. C, Nanomaterials and interfaces·2015

Area of Science:

  • Toxicology
  • Hepatology
  • Pharmacology

Background:

  • Cocaine is a potent stimulant with known cardiotoxic effects.
  • Hepatotoxicity following cocaine use is a recognized clinical concern.
  • The precise mechanisms and timeline of cocaine-induced liver injury require further elucidation.

Purpose of the Study:

  • To investigate the temporal relationship between cocaine administration and the onset of liver damage in a murine model.
  • To assess biochemical markers of liver injury and cellular integrity following cocaine exposure.
  • To examine the role of cytochrome P-450 and lipid peroxidation in cocaine-induced hepatotoxicity.

Main Methods:

  • DBA/2Ha mice were treated with phenobarbitone followed by cocaine administration.
  • Liver tissue and plasma samples were collected at 2 and 3 hours post-cocaine administration.

Related Experiment Videos

  • Histopathological examination assessed peripheral portal cell necrosis and plasma membrane integrity.
  • Liver microsomes were analyzed for total cytochrome P-450 and malondialdehyde (MDA) levels.

    • Main Results:

    • Scattered peripheral portal cell necrosis was observed within 2 hours of cocaine administration.
    • Widespread hepatic lesions and compromised plasma membrane integrity were evident by 3 hours.
    • Plasma levels of liver enzymes showed a 15-fold increase at 3 hours.
    • No significant changes in total cytochrome P-450 levels or correlation between MDA and liver damage were found.

    Conclusions:

    • Cocaine induces rapid and significant liver damage in mice, manifesting as early as 2 hours post-administration.
    • The observed hepatotoxicity is not directly correlated with alterations in total cytochrome P-450 or lipid peroxidation.
    • These findings underscore the urgency of medical intervention in cases of suspected cocaine overdose due to the swift onset of liver injury.