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Tying C' ends of H2A and H2B using a molecular glue, tissue-type transglutaminase

Hyon E Choy1, Jae-Hong Kim, Sang Chul Park

  • 1Genome Center for Enteropathogenic Bacteria, Department of Microbiology, Chonnam National University Medical College, Dong-Gu, Hak-1-Kwang-Ju 501-746, South Korea.

Methods in Enzymology
|January 10, 2004
PubMed
Abstract

No abstract available in PubMed .

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...