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Related Experiment Videos

Peroxynitrite does not impair pulmonary and systemic vascular responses.

B D Nossaman1, P A Dabisch, J T Liles

  • 1Department of Anesthesiology, Tulane University Health Science Center, New Orleans, Louisiana 70112, USA.

Journal of Applied Physiology (Bethesda, Md. : 1985)
|January 13, 2004
PubMed
Summary

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Peroxynitrite (ONOO-) significantly impacts vascular tone, causing vasodilation in some areas and vasoconstriction in others. Its effects are rapid and repeatable, independent of cyclooxygenase pathways.

Area of Science:

  • Cardiovascular Physiology
  • Vascular Pharmacology
  • Reactive Nitrogen Species Biology

Background:

  • Peroxynitrite (ONOO-) is a reactive nitrogen species implicated in various physiological and pathological processes.
  • Understanding ONOO-'s role in vascular regulation is crucial for cardiovascular health.
  • Previous studies suggest complex interactions between ONOO- and vascular tone.

Purpose of the Study:

  • To investigate the effects of peroxynitrite (ONOO-) on systemic, hindquarters, and pulmonary vascular beds.
  • To determine the dose-dependency, onset, duration, and repeatability of ONOO- vascular responses.
  • To elucidate the mechanisms underlying ONOO-'s vascular actions, including potential cyclooxygenase involvement.

Main Methods:

  • Administered intravenous ONOO- injections in rats to assess systemic arterial pressure.

Related Experiment Videos

  • Infused ONOO- into the hindquarters vascular bed and rat lung perfusion circuit to measure perfusion pressures.
  • Evaluated the impact of repeated ONOO- exposure on responses to vasodilators, vasoactive agonists, and hypoxic pulmonary vasoconstriction.
  • Tested the effects of sodium nitrate, nitrite, and decomposed ONOO-.
  • Utilized a cyclooxygenase inhibitor to assess its influence on ONOO- mediated responses.

    • Main Results:

    • ONOO- injections decreased systemic arterial pressure and hindquarters perfusion pressure in a dose-dependent manner.
    • ONOO- administration increased pulmonary arterial perfusion pressure in the isolated perfused rat lung.
    • Vascular responses to ONOO- were rapid, short-lived, and repeatable without tachyphylaxis.
    • Repeated ONOO- exposure did not alter responses to endothelium-dependent vasodilators or other vasoactive agents.
    • ONOO- effects were independent of cyclooxygenase activity and hypoxic pulmonary vasoconstriction.

    Conclusions:

    • Peroxynitrite exhibits potent pulmonary vasoconstrictor, systemic vasodepressor, and vasodilator activities.
    • Short-term, repeated exposure to ONOO- can modulate vascular responsiveness.
    • The vascular actions of ONOO- are not mediated by cyclooxygenase products.