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Impact of DNA polymerases and their buffer systems on quantitative real-time PCR
Petra Wolffs1, Halfdan Grage, Oskar Hagberg
1Applied Microbiology, Lund Institute of Technology, Mathematical Statistics, Lund University, SE-221 00 Lund, Sweden.
Journal of Clinical Microbiology
|January 13, 2004
Abstract:
An investigation of the influence of five DNA polymerase-buffer systems on real-time PCR showed that the choice of both DNA polymerase and the buffer system affected the amplification efficiency as well as the detection window. The analytical repeatability of the data for different systems changed clearly, leading us to conclude that basing quantitative measurements on single-data-set standard curves can lead to significant errors.