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Updated: Aug 3, 2026

Lateral Root Inducible System in Arabidopsis and Maize
Published on: January 15, 2016
Nitric oxide plays a central role in determining lateral root development in tomato
Natalia Correa-Aragunde1, Magdalena Graziano, Lorenzo Lamattina
1Instituto de Investigaciones Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, CC 1245, 7600, Mar del Plata, Argentina.
Nitric oxide (NO) promotes lateral root development in plants, an auxin-dependent process. This study confirms NO
Area of Science:
- Plant Physiology
- Molecular Biology
- Biochemistry
Background:
- Nitric oxide (NO) is a key signaling molecule in plants.
- Auxin is the primary hormone regulating root architecture.
- The interplay between NO and phytohormones is crucial for plant development.
Purpose of the Study:
- To investigate the role of nitric oxide (NO) in regulating lateral root (LR) development.
- To determine if NO acts in conjunction with auxin signaling pathways.
- To elucidate the physiological significance of endogenous NO in root growth.
Main Methods:
- Application of NO donor (sodium nitroprusside, SNP) and NO scavenger (CPTIO) to tomato seedlings.
- Measurement of primary root (PR) and lateral root (LR) growth.
- Detection of endogenous NO using DAF-2 DA probe.
- Manipulation of auxin levels using auxin transport inhibitor (NPA) and synthetic auxin (NAA).
Main Results:
- SNP application increased LR emergence and elongation while reducing PR growth.
- CPTIO blocked SNP's effects and inhibited endogenous LR emergence, increasing PR length.
- NO signals were localized in LR primordia.
- SNP promoted LR development even in auxin-depleted conditions, and CPTIO inhibited NAA-induced LR formation.
Conclusions:
- Nitric oxide (NO) plays a significant role in promoting lateral root development.
- NO likely functions within the auxin signaling transduction pathway.
- Endogenous NO is essential for regulating root system architecture in plants.
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