Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Interdomain communication between weak structural elements within a disease-related human tRNA.

Marc D Roy1, Lisa M Wittenhagen, Brian E Vozzella

  • 1Boston College, Eugene F. Merkert Chemistry Center, Chestnut Hill, Massachusetts 02467, USA.

Biochemistry
|January 14, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integrating Transcription Factors with Electrochemical Pendulum Bioanalysis for Hormone Detection.

Journal of the American Chemical Society·2026
Same author

Biomolecular Condensates as Protein Degradation Tools for Intracellular Targets.

Nature communications·2026
Same author

Resilient nanostructured bioanalytic microneedle longitudinally monitors preclinical renal and hepatic drug clearance and dysfunction.

Science translational medicine·2026
Same author

Amino acid supplementation enhances in vivo efficacy of lipid nanoparticle-mediated mRNA delivery in preclinical models.

Science translational medicine·2026
Same author

Toward Reagentless and Universal Biomolecular Sensing: Molecular Pendulum-Based Bioanalysis.

Accounts of chemical research·2025
Same author

Wearable biomolecular sensing nanotechnologies in chronic disease management.

Nature nanotechnology·2025
Same journal

Aromatic Cage-Directed Azide-Methyllysine Photochemistry for Profiling Nonhistone Interacting Partners of the MeCP2 Methyl-CpG-Binding Domain.

Biochemistry·2026
Same journal

Differential Hydroxypyruvate Processing by <i>E. coli</i> and <i>P. aeruginosa</i> DXP Synthases Reveals Preferential Xylulose 5-Phosphate Formation by the <i>P. aeruginosa</i> Enzyme.

Biochemistry·2026
Same journal

Structural and Functional Characterization of Heterologous Nitrogenase Complexes.

Biochemistry·2026
Same journal

Discovery of Bacterial Unspecific Peroxygenases.

Biochemistry·2026
Same journal

Lactate Biology: Subcellular Routing and Chemical Form Define Function.

Biochemistry·2026
Same journal

Nature's Anaerobic Toolkit: Glycyl Radical Enzymes and Their Expanding Functional and Mechanistic Diversity.

Biochemistry·2026
See all related articles

Mitochondrial tRNALeu(UUR) instability links disease mutations to dysfunction. Stabilizing key structural domains restored function, revealing how propagated weaknesses impact tRNA activity.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • Human mitochondrial tRNALeu(UUR) possesses domains with limited thermodynamic stability.
  • Disease-associated mutations frequently occur in these unstable regions, suggesting a link between structure and pathogenicity.

Purpose of the Study:

  • To investigate the structural basis of human mitochondrial tRNALeu(UUR) instability.
  • To determine the relationship between structural stability, mutations, and tRNA function.

Main Methods:

  • Nuclease probing was employed to map the structure of wild-type and mutant tRNALeu(UUR).
  • Mutations were introduced into the D and anticodon stems to alter stability.
  • Aminoacylation activity assays were performed to assess tRNA function.

Related Experiment Videos

Main Results:

  • Wild-type mitochondrial tRNALeu(UUR) exhibited partial denaturation in key domains.
  • Mutations stabilizing the D and anticodon stems increased structural integrity.
  • Increased structural order in D stem mutants correlated with decreased aminoacylation activity.
  • Stabilizing the D stem rescued the function of a pathogenic anticodon stem mutant.

Conclusions:

  • The inherent structural flexibility of mitochondrial tRNALeu(UUR) is crucial for its function.
  • Propagated structural weaknesses, initiated by mutations, contribute to tRNA functional deficits.
  • Interdomain communication highlights the complex structural regulation of tRNA activity.