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Published on: April 22, 2016
Immobilization of Pseudomonas delafieldii with magnetic polyvinyl alcohol beads and its application in
Guo-Bin Shan1, Jian-Min Xing, Ming-Fang Luo
1Laboratory of Separation Science and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, PR China.
Abstract:
Pseudomonas delafieldii was immobilized in magnetic polyvinyl alcohol (PVA) beads using a hydrophilic magnetic fluid, which was prepared by a co-precipitation method. The beads had distinct super-paramagnetic properties and were compared with immobilized cells in non-magnetic PVA beads. Their desulfurizing activity was increased slightly from 8.7 to 9 mmol sulfur kg(-1) (dry cell) h(-1). The main advantages was that the magnetic immobilized cells maintain a high desulfurization activity and remain in good shape after 7 times of repeated use, while the non-magnetic immobilized cells could only be used for 5 times. Furthermore, the magnetic immobilized cells could be easily collected or separated magnetically from the biodesulfurization reactor.
