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Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis
Published on: June 10, 2020
[Cloning and sequence analysis of Chlamydia trachomatis heat shock protein 10]
1Clinical Laboratory, Nanjing Yuhua Hospital, Nanjing, Jiangsu 210012, China.
Objective:
To obtain Chlamydia trachomatis heat shock protein (cHSP) 10 gene from clinical secretion samples.
Methods:
cHSP10 gene was amplified from 20 cases of clinical secretion samples with positive gold-labelling by specific primers of cHSP10 and identified by sequence analysis.
Results:
cHSP10 full-length gene was amplified from 1 of 20 cases of clinical secretion samples with positive gold-labelling. cHSP10 gene encoding 102 amino acids contains 306 bp, which nuclotide at position 194 changes from T to A, leading to the change of corresponding amino acid.
Conclusions:
cHSP10 gene may be cloned from clinical secretion samples with positive gold-labelling, which make it possible to further construct expression plasmid of recombinant cHSP10.
