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Cryo-electron Microscopy Specimen Preparation By Means Of a Focused Ion Beam
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Preparation of cross-section specimens for transmission electron microscopy from alpha-Al2O3/Ni solid state bonded

C Wan1, M Dupeux

  • 1Laboratoire de Thermodynamique et Physico-Chimie Métallurgiques, Domaine Universitaire, Saint Martin d'Hères, France.

Microscopy Research and Technique
|November 1, 1992
PubMed

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Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...