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Published on: March 18, 2012
Coenzyme specificity of enzymes in the oxidative pentose phosphate pathway of Gluconobacter oxydans
Naoto Tonouchi1, Masakazu Sugiyama, Kenzo Yokozeki
1AminoScience Laboratories, Ajinomoto Co., Inc., Kawasaki-shi, Japan.
Abstract:
The coenzyme specificity of enzymes in the oxidative pentose phosphate pathway of Gluconobacter oxydans was investigated. By investigation of the activities of glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6PGDH) in the soluble fraction of G. oxydans, and cloning and expression of genes in Escherichia coli, it was found that both G6PDH and 6PGDH have NAD/NADP dual coenzyme specificities. It was suggested that the pentose phosphate pathway is responsible for NADH regeneration in G. oxydans.
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