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Updated: Aug 29, 2026

Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE Immunoblotting
10:51

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Optimization of the first dimension for separation by two-dimensional gel electrophoresis of basic proteins from

Kyla Pennington1, Emma McGregor, Clare L Beasley

  • 1Department of Psychological Medicine, Institute of Psychiatry, UK. k.pennington@iop.kcl.ac.uk

Proteomics
|January 20, 2004
PubMed

A major cause of poor resolution in the alkaline pH range of two-dimensional electrophoresis (2-DE) gels is unsatisfactory separation of basic proteins in the first dimension. We have compared methods for the separation of basic proteins in the isoelectric focusing dimension of human brain proteins. The combined use of anodic cup-loading and the hydroxyethyldisulphide containing solution (DeStreak) produced better resolution in both analytical and micropreparative protein loaded 2-DE gels than the other methods investigated.

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