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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
pre-mRNA Processing02:01

pre-mRNA Processing

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps the cell...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...

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Updated: Jun 27, 2026

Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy
11:40

Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy

Published on: June 25, 2013

Recombination and RNA processing: a common strand?

S Kearsey1, D Kipling

  • 1Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, UK.

Trends in Cell Biology
|November 1, 1991
PubMed
Summary
This summary is machine-generated.

Genetic recombination is vital for altering genome structure. A RecA-like protein in yeast, Saccharomyces cerevisiae, shows unexpected biochemical activities beyond recombination, suggesting broader cellular roles.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Cellular Biology

Background:

  • Genetic recombination is a fundamental cellular process essential for genome integrity and evolution.
  • The RecA protein from Escherichia coli is a key mediator of homologous recombination.
  • A eukaryotic homolog of RecA has been identified in yeast, Saccharomyces cerevisiae.

Purpose of the Study:

  • To investigate the biochemical properties of the RecA-like protein in Saccharomyces cerevisiae.
  • To determine if the function of this eukaryotic RecA-like protein is solely limited to recombination.

Main Methods:

  • Biochemical assays were performed to characterize the enzymatic activities of the yeast RecA-like protein.
  • Comparative analysis with E. coli RecA was conducted.

Main Results:

  • The yeast RecA-like protein exhibits biochemical activities beyond those typically associated with homologous recombination.
  • These additional activities suggest a potentially wider range of functions for this protein within the cell.

Conclusions:

  • The RecA-like protein in Saccharomyces cerevisiae possesses multifaceted biochemical capabilities.
  • Its functional role may extend beyond DNA repair and recombination, impacting other cellular processes.