Vesicle budding: insights from cell-free assays

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Updated: Aug 29, 2026

The Encapsulation of Cell-free Transcription and Translation Machinery in Vesicles for the Construction of Cellular Mimics
10:28

The Encapsulation of Cell-free Transcription and Translation Machinery in Vesicles for the Construction of Cellular Mimics

Published on: October 21, 2013

P Melançon1, A Franzusoff, K E Howell

  • 1Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309, USA.

Trends in Cell Biology
|December 1, 1991
PubMed

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Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...