Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Early events in eukaryotic DNA replication.

J F Diffley1

  • 1Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts, UK EN6 3LD.

Trends in Cell Biology
|October 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Coping with and recovering from hydroxyurea-induced replication fork arrest in budding yeast.

Cold Spring Harbor symposia on quantitative biology·2003
Same author

Mrc1 transduces signals of DNA replication stress to activate Rad53.

Nature cell biology·2001
Same author

MCM2-7 proteins are essential components of prereplicative complexes that accumulate cooperatively in the nucleus during G1-phase and are required to establish, but not maintain, the S-phase checkpoint.

Molecular biology of the cell·2001
Same author

Separate SCF(CDC4) recognition elements target Cdc6 for proteolysis in S phase and mitosis.

The EMBO journal·2001
Same author

Human origin recognition complex binds to the region of the latent origin of DNA replication of Epstein-Barr virus.

The EMBO journal·2001
Same author

Regulation of DNA replication fork progression through damaged DNA by the Mec1/Rad53 checkpoint.

Nature·2001
Same journal

Horizontal transfer of mitochondria in cancer: The physiology reborn in disease?

Trends in cell biology·2026
Same journal

Spindle errors: A stress test for epithelial robustness.

Trends in cell biology·2026
Same journal

Multicellular ecosystems: Linking cellular diversity to tissue function and disease.

Trends in cell biology·2026
Same journal

Orchestrating the signaling-bias at the protease-activated receptor, PAR1.

Trends in cell biology·2026
Same journal

Crashing by design: Utilizing DNA damage for MCC differentiation.

Trends in cell biology·2026
Same journal

The value of a shared lab: Our insights.

Trends in cell biology·2026
See all related articles

Scientists identified key proteins and their interactions involved in eukaryotic DNA replication during the S phase of the cell cycle. This research advances our understanding of how DNA replication is controlled.

Area of Science:

  • Molecular Biology
  • Cell Cycle Regulation
  • Genetics

Background:

  • Eukaryotic DNA replication is a fundamental process crucial for cell division.
  • This process is tightly regulated and occurs specifically during the S phase of the cell cycle.
  • Understanding the molecular mechanisms controlling DNA replication is vital for comprehending cell proliferation and disease.

Purpose of the Study:

  • To identify key protein participants in eukaryotic DNA replication.
  • To characterize protein-protein interactions essential for forming functional replication complexes.
  • To advance the understanding of cell cycle control over DNA replication.

Main Methods:

  • Investigated DNA replication in two distinct biological systems.
  • Utilized biochemical and genetic approaches to identify key proteins.

Related Experiment Videos

  • Analyzed protein-protein interactions using established molecular biology techniques.

    • Main Results:

    • Identified novel key participants in the DNA replication machinery.
    • Characterized numerous essential protein-protein interactions within replication complexes.
    • Provided insights into the assembly and function of replication machinery.

    Conclusions:

    • Significant progress has been made in identifying the molecular players of eukaryotic DNA replication.
    • The characterization of protein interactions sheds light on the regulation of replication initiation and progression.
    • A clearer understanding of cell cycle control over DNA replication is emerging.