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Updated: Aug 10, 2026

Identification of Kinase-substrate Pairs Using High Throughput Screening
Published on: August 29, 2015
Ste5: a meeting place for MAP kinases and their associates
1Dept of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA.
Abstract:
Growth and differentiation of the budding yeast Saccharomyces cerevisiae is regulated by six functionally distinct but structurally similar MAP kinase cascades. Three of the protein kinases in the cascade that regulates G1-phase arrest and mating have recently been shown to form a multikinase complex with a LIM-domain-containing protein called Ste5. These studies implicate Ste5 as a tethering protein that physically links protein kinases operating sequentially in a cascade. The significance of this complex for the regulation and specificity of signal transduction is explored in this review.
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