Related Experiment Video
Updated: Aug 29, 2026
Rapid Quantification of Oxidized and Reduced Forms of Glutathione Using Ortho -phthalaldehyde in Cultured Mammalian Cells In Vitro
Published on: June 28, 2024
Relationship between malondialdehyde level and glutathione peroxidase activity in diabetic rats
Durdi Qujeq1, Hamid Reza Aliakbarpour, Khodaberdi Kalavi
1Department of Biochemistry and Biophysics, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran. dqujeq@hotmail.com
Background:
This investigation describes the relationship between glutathione peroxidase activities, an antioxidant enzyme, and the oxidative status in diabetic rat blood.
Methods:
Malondialdehyde level and glutathione peroxidase activity were measured by spectrophotometric techniques.
Results And Conclusions:
Malondialdehyde content in the diabetic rats group was increased compared to that in the controls [3.08+/-0.32 (mean+/-S.E.) vs. 1.15+/-0.29 mM/g hemoglobin, P>0.01]. Glutathione peroxidase activity in the diabetic rats group was increased compared to that in the control [10.27+/-1.39 (mean+/-S.E.) vs. 3.14+/-0.38 micromol NADPH/min/g hemoglobin, P>0.01]. Our results show a positive correlation between serum glutathione peroxidase and malondialdehyde concentration in diabetic rats.
