Cloning genes from a library using a clustering strategy and PCR
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S Díaz Prado1, N Tarrío, M E Cerdán
1Department of Molecular and Cell Biology, University of A Coruña, Campus da Zapateira s/n, 15071-A Coruña, Spain.
Molecular Biotechnology
|January 22, 2004
Abstract:
A new polymerase chain reaction (PCR)-based method is described for the isolation of clones of interest from a library when only part of a sequence is available. In actuality, this occurs with many genomes that have been partially sequenced using a random strategy. The method presented here, discriminating clusters by PCR (DCbyPCR), is a nonradioactive and improved alternative to colony hybridization.

