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On-chip electrochemical measurement of beta-galactosidase expression using a microbial chip
Takatoshi Kaya1, Kuniaki Nagamine, Nobuto Matsui
1Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Sendai, Japan.
Chemical Communications (Cambridge, England)
|January 23, 2004
Abstract:
[small beta]-Galactosidase expression in a small number of Escherichia coli cells has been measured in real time with an electrochemical sensor chip. E. coli cells were embedded using collagen gel within a micropore which was microfabricated onto a chip. The activity of the expressed [small beta]-galactosidase was determined using p-aminophenyl [small beta]-d-galactopyranoside (PAPG) as a substrate.