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[Recommended techniques for obtaining nail specimens and mycologic diagnosis of onychomycosis]

F Foulet1, G Cremer

  • 1Consultation de Dermato-Mycologie, Hôpital Henri-Mondor, 51, avenue du Maréchal-de-Lattre-de-Tassigny, 94010 Créteil, France.

Annales De Dermatologie Et De Venereologie
|January 27, 2004
PubMed

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Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...