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Related Experiment Videos

Postnatal gene expression in the normal mouse cornea by SAGE.

Barbara Norman1, Janine Davis, Joram Piatigorsky

  • 1Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.

Investigative Ophthalmology & Visual Science
|January 28, 2004
PubMed
Summary

This study profiles gene expression in mouse corneas at postnatal day 9 and adulthood. It reveals dynamic changes after eye opening, identifying new markers for corneal epithelial cell stratification and function.

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Area of Science:

  • Ophthalmology
  • Molecular Biology
  • Genomics

Background:

  • The cornea's cellular and molecular architecture is crucial for its transparency and function.
  • Understanding gene expression patterns in the developing and mature cornea is essential for diagnosing and treating ocular diseases.

Purpose of the Study:

  • To generate a comprehensive gene expression profile of the normal postnatal mouse cornea at two key developmental stages.
  • To identify genes involved in corneal development, stratification, and function.

Main Methods:

  • Serial Analysis of Gene Expression (SAGE) was employed on postnatal day 9 (PN9) and adult (6-week-old) mouse corneas.
  • In situ hybridization was used to analyze the expression patterns of selected genes.
  • Transcriptome analysis identified unique and differentially expressed messenger RNAs (mRNAs).

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Main Results:

  • Sequencing yielded over 64,000 tags for PN9 and 62,000 for adult corneas, revealing approximately 19,500 and 18,500 unique mRNAs, respectively.
  • A significant portion of transcripts (one-third) were common to both stages, while others were stage-specific.
  • Enriched transcripts were associated with metabolic functions, redox activity, and barrier integrity. Specific genes like Aquaporin 5 and glutathione-S-transferase (GST) variants were identified as markers for distinct corneal epithelial layers.

Conclusions:

  • The study provides a detailed gene expression profile of the mouse cornea, highlighting dynamic changes post-eye opening.
  • The identified genes serve as novel probes for investigating corneal epithelial cell stratification, development, and function.
  • These findings offer insights into the interplay between programmed and environmentally influenced gene expression in the cornea.