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Updated: Jul 30, 2026

A Simple Fecal Flotation Method for Diagnosing Zoonotic Nematodes Under Field and Laboratory Conditions
Published on: December 15, 2023
[Intestinal parasitosis in the asymptomatic Subsaharian immigrant population. Gran Canaria 2000]
A M Martín Sánchez1, A Hernández García, M González Fernández
1Servicio de Microbiología, Hospital Universitario Insular de Gran Canaria, Universidad de Las Palmas de Gran Canaria. mmartin@cicei.ulpgc.es
Context:
A prospective study has been conducted in order to known the prevalence and the type of intestinal parasitoses in a cohort of healthy Subsaharian immigrants that reached Gran Canaria along the year 2000.
Methods:
348 fecal samples of 121 immigrants have been studied. In each patient a minimum of two samples and a maximum of three samples was studied. Each sample was evaluated through two techniques: visualization of a direct preparation dyed with Lugol and through the technique of concentration of Kato.
Results:
23.1% of the patients presented pathogenic parasites in feces, being observed 2 parasites in 5 of the patients (17.8%). The geohelminths (Ancylostoma duodenale/Necator americanus, Ascaris lumbricoides, Trichuris trichiura and Strongyloides stercolaris) were the parasites more frequently found (87.9%). Specifically, the parasitosis found in the greatest number of participants was hookworm disease (44.8%). In this study the effectiveness of the evaluation of three feces samples is demonstrated in order to increase the percentage of diagnosed intestinal parasitoses. The visualization of a direct preparation dyed with Lugol permitted the diagnosis of 63.6% of the parasitoses, while the technique of Kato detected 56.6% of the helminth infections.
Conclusions:
The prevalence of geohelminths in the asymptomatic Subsaharian immigrant population is elevated. The complementary use of the microscopic study of a direct preparation dyed with Lugol and the technique of Kato increase the diagnostic performance.
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