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Related Experiment Videos

Bacteriolysis by immobilized enzymes.

I Karube, T Suganuma, S Suzuki

    Biotechnology and Bioengineering
    |March 1, 1977
    PubMed
    Summary

    Achromobacter lunatus bacteriolytic enzymes immobilized on collagen membranes effectively lyzed intact bacteria. The immobilized enzymes showed enhanced stability against sodium chloride, an inhibitor of native enzymes.

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    Area of Science:

    • Biochemistry
    • Microbiology
    • Biotechnology

    Background:

    • Bacteriolytic enzymes are crucial for degrading bacterial cell walls.
    • Immobilization of enzymes can enhance their stability and reusability.
    • Achromobacter lunatus produces potent bacteriolytic enzymes.

    Purpose of the Study:

    • To immobilize bacteriolytic enzymes from Achromobacter lunatus in a collagen membrane.
    • To evaluate the efficacy of the immobilized enzymes against various intact bacteria.
    • To assess the stability and optimal conditions for the immobilized enzyme activity.

    Main Methods:

    • Enzyme immobilization using collagen membrane.
    • Bacterial lysis assays with intact bacteria (Pseudomonas solanacearum, Xanthomonas oryzae, Staphylococcus aureus, Pseudomonas aeruginosa).
    • Determination of relative enzyme activity, optimum pH, and stability against sodium chloride.

    Main Results:

    • The bacteriolytic enzyme-collagen membrane successfully lyzed intact bacteria.
    • Immobilized enzyme activity against Pseudomonas solanacearum was 2% of native enzymes.
    • Immobilized enzymes exhibited stability against sodium chloride, unlike native enzymes.
    • A continuous reactor with rolled enzyme-collagen membrane effectively lyzed Xanthomonas oryzae and Pseudomonas aeruginosa.

    Conclusions:

    • Immobilization of Achromobacter lunatus bacteriolytic enzymes in collagen membrane is feasible.
    • The immobilized enzymes retain bacteriolytic activity and show improved stability against inhibitors.
    • This immobilized enzyme system offers potential for continuous bacterial lysis applications.

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