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Odorant receptor expression in the mouse cerebral cortex.

Joji M Otaki1, Haruhiko Yamamoto, Stuart Firestein

  • 1Department of Biological Sciences, Columbia University, New York, New York 10027, USA.

Journal of Neurobiology
|January 30, 2004
PubMed
Summary
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Mammalian odorant receptors are found in the mouse cerebral cortex, not just olfactory neurons. These receptors may aid in chemical detection or neuronal development in the brain.

Area of Science:

  • Neuroscience
  • Molecular Biology

Background:

  • Mammalian odorant receptors (ORs) are primarily known for odor detection in olfactory sensory neurons.
  • ORs also play roles in the neuronal development of these sensory neurons.

Purpose of the Study:

  • To investigate the expression of odorant receptors in nonolfactory neurons within the mouse brain.
  • To explore potential novel functions of ORs beyond olfaction.

Main Methods:

  • Polymerase chain reaction (PCR) was used to detect OR gene expression in different mouse brain tissues.
  • Degenerate PCR was employed to identify novel ORs in the cerebral cortex.
  • In situ hybridization and X-gal staining in transgenic mice (M71-IRES-tauLacZ) were used to localize OR expression.

Main Results:

Related Experiment Videos

  • Specific mouse odorant receptors (M71, C6, OR3) were detected in the cerebral cortex via PCR, but not in other brain regions.
  • Degenerate PCR indicated the presence of other ORs in the cerebral cortex.
  • In situ hybridization and genetic marking confirmed M71 and C6 receptor expression in layer II pyramidal neurons in the occipital pole.
  • Expression was observed from postnatal day 3 (P3) through adulthood, with a peak at P8.

Conclusions:

  • At least a few odorant receptors are expressed in mouse cerebral cortex pyramidal neurons, in addition to olfactory sensory neurons.
  • These cortical ORs might be involved in detecting exogenous/endogenous ligands or in developmental processes like axon guidance.