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A digital method of sperm immobilization test: comparison to the conventional method
Shinji Komori1, Yukari Hamada, Akiko Hasegawa
1Department of Obstetrics and Gynecology, Hyogo College of Medicine, Nishinomiya, Hyogo, Japan. komor615@hyo-med.ac.jp
American Journal of Reproductive Immunology (New York, N.Y. : 1989)
|January 31, 2004
Summary
Antisperm antibodies linked to infertility can be objectively measured. A new digital method using Sperm Quality Analyzer provides accurate results comparable to traditional subjective tests for sperm immobilization.
Area of Science:
- Reproductive immunology
- Andrology
- Clinical diagnostics
Background:
- Antisperm antibodies are implicated in infertility, particularly those causing sperm immobilization.
- Conventional sperm immobilization tests are subjective, relying on manual microscopic assessment of sperm motility.
- Unexplained infertility may be linked to the presence of these antibodies.
Purpose of the Study:
- To evaluate a digital method for analyzing motile sperm.
- To compare the digital method's results with the conventional sperm immobilization test.
- To determine the objectivity and value of the digital method in detecting sperm immobilizing antibodies.
Main Methods:
- Analysis of motile sperm using a digital Sperm Quality Analyzer.
- Comparison of digital analysis results with a conventional microscopic sperm immobilization test.
- Testing of 66 patient samples for sperm immobilizing antibodies.
Main Results:
- The digital method and the conventional method yielded identical results.
- 14 out of 66 samples tested positive for sperm immobilizing antibodies.
- 52 out of 66 samples tested negative for sperm immobilizing antibodies.
Conclusions:
- The digital method for analyzing motile sperm is objective.
- This digital approach is valuable for the accurate determination of sperm immobilizing antibodies.
- Objective measurement can improve the diagnosis of infertility related to antisperm antibodies.